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KMID : 0368419980410020093
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Journal of Plant Biology
1998 Volume.41 No. 2 p.93 ~ p.96
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Effect of Age of Seedling and Phytohormones on Micropropagation of Indica Rice(Oryza sativa L.) from Meristem Culture
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Farzana, Khanum
Husnain, Tayyab/Riazuddin, Sheikh
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Abstract
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An efficient protocol for in vitro micropropagation of seven indica rice varieties was developed from meristem culture. Meristem (leaf base) was isolated from different age of seedlings and cultured on MS medium without hormones and supplemented with different concentrations of NAA and BAP. Regeneration of plantlets from meristem was observed within five days of culture. The meristem isolated from 4-day old seedlings gave highest regeneration on hormone free MS medium. Histological study of meristem (leaf base) from 4-day old seedlings confirmed the presence of meristematic cells. Regenerated plants were multiplied on MS medium supplemented with 0.05 ㎎/L NAA and 5 ㎎/L BAP. An average of five plants were obtained from single regenerated meristem. The plants regenerated from meristem showed morphological uniformity.
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